TB-500 is a synthetic analogue of the active region of Thymosin Beta-4 (Tb4), a 43-amino-acid actin-sequestering peptide. CAS: 77591-33-4 | Approximate MW: 4,963.5 Da. Supplied as a white lyophilised powder at ≥99% purity (HPLC-verified).

Research Background

Thymosin Beta-4 was initially characterised in thymic biology before researchers identified its central role in actin polymerisation dynamics. The TB-500 fragment has become a widely studied model compound in wound healing, angiogenesis, and cell migration research. Preclinical studies have been published examining TB-500 in cardiac, muscular, tendon, and neural tissue models.

Mechanism of Action

TB-500 binds to G-actin monomers through its LKKTET motif, modulating the G-actin:F-actin equilibrium within cells. This influences cell morphology, motility, and adhesion – fundamental to wound healing cascades. Research has also documented VEGF upregulation and angiogenic responses in animal models, alongside interactions with metalloproteinase systems in ECM remodelling.

Storage & Handling

• Long-term storage: -20°C (lyophilised powder stable for 24+ months)
• Short-term storage: 2-8°C for up to 4 weeks after reconstitution
• Reconstitution: Sterile bacteriostatic water; dissolve gently by swirling (do not vortex)
• Handling: Aliquot into single-use volumes; store away from direct light

Related Research Compounds

TB-500 is frequently studied alongside BPC-157 10mg in tissue repair research. GHK-Cu 50mg is another closely related compound for connective tissue biology research.

Frequently Asked Questions

Q: What is TB-500?

A: TB-500 is a synthetic peptide corresponding to the actin-binding region of Thymosin Beta-4 (CAS 77591-33-4, ~4,963 Da). Used in preclinical research to study actin dynamics, cell migration, and tissue repair. For research use only.

Q: How is TB-500 typically used in research?

A: Reconstituted in sterile bacteriostatic water and applied in cell culture assays or animal model experiments studying wound healing, angiogenesis, and tissue regeneration.

Q: What is the storage recommendation for TB-500?

A: -20°C lyophilised (24+ months). Reconstituted: 2-8°C, use within 4 weeks. Aliquot to avoid repeated freeze-thaw cycles.

Q: What purity and form is TB-500 supplied in?

A: White lyophilised powder at ≥99% purity (HPLC-verified). 10mg per vial.

For research use only. Not for human or animal consumption. Not a medicinal product.