5-Amino-1MQ (5-amino-1-methylquinolinium) is a selective small molecule inhibitor of nicotinamide N-methyltransferase (NNMT). CAS: 1673498-15-1 | MW: 174.20 g/mol. Supplied at ≥98% purity for metabolic biology, adipose tissue, and NAD+ metabolism research.

Research Background

NNMT catalyses the methylation of nicotinamide using S-adenosylmethionine (SAM) as the methyl donor, consuming both NAD+ precursors and methyl groups from the one-carbon cycle. NNMT expression is elevated in obesity, type 2 diabetes, and various cancers. The discovery that NNMT inhibition can increase cellular NAD+ availability and alter adipocyte differentiation in mouse models generated significant research interest. 5-Amino-1MQ emerged from structure-activity relationship studies as a potent, cell-permeable NNMT inhibitor.

Mechanism of Action & Research Applications

5-Amino-1MQ competitively inhibits NNMT by occupying the nicotinamide-binding pocket. Key preclinical research findings:

• NAD+ elevation: NNMT inhibition diverts nicotinamide back into the NAD+ biosynthetic pathway, increasing cellular NAD+ concentrations
• Adipogenesis inhibition: Studies in mouse adipocytes show inhibition of adipocyte differentiation and lipid accumulation
• SAM conservation: Reduced SAM consumption may increase available methyl groups for epigenetic methylation reactions
• Metabolic rate effects: Animal studies report effects on body composition and energy expenditure in obesity models

Storage & Handling

• Long-term storage: -20°C, protected from light and moisture
• Short-term storage: 2-8°C for up to 2 weeks in working solution
• Reconstitution: Soluble in DMSO; prepare stock solutions in DMSO and dilute for cell culture (≤0.1% DMSO final)

Related Research Compounds

Closely related to NAD+ biology: NAD+ 500mg, MOTS-C 40mg (AMPK activator), and SS-31 10mg.

For research use only. Not for human or animal consumption. Not a medicinal product.